Chiu Lab Publications
2024
Stella Y Sun, Li-Av Segev-Zarko, Grigore D Pintilie, Chi Yong Kim, Sophia R Staggers, Michael F Schmid, Elizabeth S Egan, Wah Chiu, John C Boothroyd (10 April 2024) Cryogenic electron tomography reveals novel structures in the apical complex of Plasmodium falciparum. mBio | Paper | Link |
David Chmielewski, Guan-Chin Su, Jason T Kaelber, Grigore D Pintilie, Muyuan Chen, Jing Jin, Albert J Auguste, Wah Chiu (07 March 2024) Cryogenic electron microscopy and tomography reveal imperfect icosahedral symmetry in alphaviruses. PNAS Nexus | Paper | Link |
Rachael C Kretsch, Lily Xu, Ivan N Zheludev, Xueting Zhou, Rui Huang, Grace Nye, Shanshan Li, Kaiming Zhang, Wah Chiu, Rhiju Das (5 March 2024) Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses. Proc Natl Acad Sci U S A | Paper | Link |
Mengyuan Xu, Torben Neelands, Alexander S. Powers, Yan Liu, Steven D Miller, Grigore D Pintilie, J Du Bois, Ron O Dror, Wah Chiu, Merritt Maduke (12 February 2024) CryoEM structures of the human CLC-2 voltage-gated chloride channel reveal a ball-and-chain gating mechanism. Elife | Paper | Link |
2023
Momei Zhou, Benjamin Vollmer, Emily Machala, Muyuan Chen, Kay Grunewald, Ann M. Arvin, Wah Chiu, Stefan L. Oliver (02 December 2023) Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states. Nature Communications | Paper | Link |
Muyuan Chen, Michael F. Schmid, Wah Chiu (16 November 2023) Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models. Nature Methods | Paper | Link |
David Chmielewski, Eric A. Wilson, Grigore Pintilie, Peng Zhao, Muyuan Chen, Michael F. Schmid, Graham Simmons, Lance Wells, Jing Jin, Abhishek Singharoy, Wah Chiu (07 November 2023) Structural insights into the modulation of coronavirus spike tilting and infectivity by hinge glycans. Nature Communications | Paper | Link |
Martin A. Walsh, Brent L. Nannenga, Tamir Gonen, Patrick M. Sexton, Denise Wooten, Wah Chiu, Fei Sun, Bridget Carragher, Clinton S. Potter, David Agard, Stephen K. Burley (2 November 2023) The next decade in structural biology. Structure | Paper | LInk |
Rachael C. Kretsch, Ebbe S. Andersen, Janusz M. Bujnicki, Wah Chiu, Rhiju Das, Bingnan Luo, Benoit Masquida, Ewan K.S. McRae, Griffin M. Schroeder, Zhaoming Su, Joseph E. Wedekind, Lily Xu, Kaiming Zhang, Ivan N. Zheludev, John Moult, Andriy Kryshtafovych (19 July 2023) RNA target highlights in CASP15: Evaluation of predicted models by structure providers. Proteins | Paper | Link |
Hirenkumar R Patel, Joel Imventarza, Ajay Kumar, Ali Shariati, Chinyere A Iweka, Aditi Swarup, Karanvir Kaushal, Shweta Modgil, Shou Li, Michael Nahmou, Gong-Her Wu, Wah Chiu, Yaping J. Liao (June 2023) Development of human cellular model for ectopic calcification to study the physiopathological mechanism for Optic Disc Drusen (ODD). Investigative Ophthalmology & Visual Science | Link
Natasha K Dudek, Jesus G Galaz-Montoya, Handuo Shi, Megan Mayer, Cristina Danita, Arianna I Celis, Tobias Viehboeck, Gong-Her Wu, Barry Behr, Silvia Bulgheresi, Kerwyn Casey Huang, Wah Chiu, David A Relman (13 April 2023) Previously uncharacterized rectangular bacterial structures in the dolphin mouth. Nature Communications | Paper | Link |
Gong-Her Wu, Chharlene Smith-Geater, Jesús G Galaz-Montoya, Yingli Gu, Sanket R Gupte, Ranen Aviner, Patrick G Mitchell, Joy Hsu, Ricardo Miramontes, Keona Q Wang, Nicolette R Geller, Cathy Hou, Cristina Danita, Lydia-Marie Joubert, Michael F Schmid, Serena Yeung, Judith Frydman, William Mobley, Chengbiao Wu, Leslie M Thompson, Wah Chiu (8 February 2023) CryoET reveals organelle phenotypes in huntington disease patient iPSC-derived and mouse primary neurons. Nature Communications. | Paper | Link
Molly F Parsons, Matthew F Allan, Shanshan Li, Tyson R Shepherd, Sakul Ratanalert, Kaiming Zhang, Krista M Pullen, Wah Chiu, Silvi Rouskin, Mark Bathe (24 January 2023) 3D RNA-scaffolded wireframe origami. Nature Communications. | Paper | Link
Shanshan Li, Kaiming Zhang, Wah Chiu (2023) Near-Atomic Resolution Cryo-EM Image Reconstruction of RNA. Methods in Molecular Biology. | Paper | Link
2022
Daniel Gestaut, Yanyan Zhao, Junsun Park, Boxue Ma, Alexander Leitner, Miranda Collier, Grigore Pintilie, Soung-Hun Roh, Wah Chiu, Judith Frydman (8 December 2022) Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT. Cell. | Press Release | Paper | Link
Stephen K Burley, Helen M Berman, Wah Chiu, Wei Dai, Justin W Flatt, Brian P Hudson, Jason T Kaelber, Sagar D Khare, Arkadiusz W Kulczyk, Catherine L Lawson, Grigore D Pintilie, Andrej Sali, Brinda Vallat, John D Westbrook, Jasmine Y Young, Christine Zardecki (2 December 2022) Electron microscopy holdings of the Protein Data Bank: the impact of the resolution revolution, new validation tools, and implications for the future. Biophysical Reviews. | Paper | Link |
Jason T Kaelber, David Chmielewski, Wah Chiu, Albert J Auguste (27 November 2022) Alphavirus Particles Can Assemble with an Alternate Triangulation Number. Viruses | Paper | Link |
Shanshan Li, Kaiming Zhang, Wah Chiu (14 October 2022) Near-Atomic Resolution Cryo-EM Image Reconstruction of RNA. Methods in Molecular Biology | Paper | Link |
Peter D Dahlberg, Davis Perez, Corey W Hecksel, Wah Chiu, W E Moerner (1 October 2022) Metallic support films reduce optical heating in cryogenic correlative light and electron tomography. Journal of Structural Biology | Paper | Link |
Cristina Danita, Wah Chiu, Jesus G Galaz-Montoya (16 September 2022) Efficient manual annotation of cryogenic electron tomograms using IMOD. STAR Protocols. | Paper | Link
Shanshan Li, Michael Z Palo, Grigore Pintilie, Xaiojing Zhang, Zhaoming Su, Kalli Kappel, Wah Chiu, Kaiming Zhang, Rhiju Das (6 September 2022) Topological crossing in the misfolded Tetrahymena ribozyme resolved by cryo-EM. Proceedings of the National Academy of Sciences. | Paper | Link
Li-av Segev-Zarko, Peter D Dahlberg, Stella Y Sun, Daniel M Pelt, Chi Yong Kim, Elizabeth S Egan, James A Sethian, Wah Chiu, John C Boothroyd (4 September 2022) Cryo-electron tomography with mixed-scale dense neural networks reveals key steps in deployment of Toxoplasma invasion machinery. PNAS Nexus | Paper | Link |
Abigail E Powell, Duo Xu, Gillie A Roth, Kaiming Zhang, Wah Chiu, Eric A Appel, Peter S Kim (24 August 2022) Multimerization of Ebola GPΔmucin on protein nanoparticle vaccines has minimal effect on elicitation of neutralizing antibodies. Frontiers in Immunology. | Paper | Link |
David Chmielewski, Michael F Schmid, Graham Simmons, Jing Jin, Wah Chiu (30 June 2022) Chikungunya virus assembly and budding visualized in situ using cryogenic electron tomography. Nature Microbiology. | Paper | Link
Xiao Wang, Shanshan Li, Hyungmin Jun, Torsten John, Kaiming Zhang, Hannah Fowler, Jonathan PK Doye, Wah Chiu, Mark Bathe (20 May 2022) Planar 2D wireframe DNA origami. Science advances. | Paper | Link
Wesley M Tierney, Ian A Vicino, Stella Y Sun, Wah Chiu, Esteban A Engel, Matthew P Taylor, Ian B Hogue (13 April 2022) Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses. Methods in Molecular Biology | Paper | Link
Yan Wang, Jennifer M Binning, Grigore D Pintilie, Wah Chiu, Gaya K Amarasinghe, Daisy W Leung, Zhaoming Su (18 March 2022) Cryo-EM analysis of Ebola virus nucleocapsid-like assembly. STAR protocols. | Paper | Link
Ashwin Balaji, Peter D Dahlberg, Li-av Segev-Zarko, Stella Sun, Wah Chiu, John Boothroyd, William E Moerner (11 February 2022) Characterizing the distribution of myosin H in the apical complex of conoid protruded and conoid retracted Toxoplasma gondii. Biophysical Journal. | Paper | Link
Kaiming Zhang, Naoki Horikoshi, Shanshan Li, Alexander S Powers, Mikhail A Hameedi, Grigore D Pintilie, Hee-Don Chae, Yousuf A Khan, Carl-Mikael Suomivuori, Ron O Dror, Kathleen M Sakamoto, Wah Chiu, Soichi Wakatsuki (7 February 2022) Cryo-EM, Protein Engineering, and Simulation Enable the Development of Peptide Therapeutics against Acute Myeloid Leukemia. American Chemical Society. | Paper | Link
Stella Y Sun, Li-av Segev-Zarko, Muyuan Chen, Grigore D Pintilie, Michael F Schmid, Steven J Ludtke, John C Boothroyd, Wah Chiu (4 February 2022) Cryo-ET of Toxoplasma parasites gives subnanometer insight into tubulin-based structures. Proceedings of the National Academy of Sciences. | Paper | Link
Zewen Zhang, Yuzhang Li, Rong Xu, Weijiang Zhou, Yanbin Li, Solomon T Oyakhire, Yecun Wu, Jinwei Xu, Hansen Wang, Zhiao Yu, David T Boyle, William Huang, Yusheng Ye, Hao Chen, Jiayu Wan, Zhenan Bao, Wah Chiu, Yi Cui (6 January 2022) Capturing the swelling of solid-electrolyte interphase in lithium metal batteries. Science. | Paper | Link
Weijiang Zhou, Benjamin Bammes, Patrick G Mitchell, Kerry Betz, Wah Chiu (January 2022) Electron crystallography of chiral and non-chiral small molecules. Ultramicroscopy. | Paper | Link
2021
Hyungmin Jun, Xiao Wang, Molly F Parsons, William P Bricker, Torsten John, Shanshan Li, Steve Jackson, Wah Chiu, Mark Bathe (2021) Rapid prototyping of arbitrary 2D and 3D wireframe DNA origami. Nucleic Acids Research. | Paper | Link
Grigore Pintilie, Wah Chiu (2021) Validation, analysis and annotation of cryo-EM structures. Acta Crystallographica Section D: Structural Biology. | Paper | Link
Peter J Blanco Carcache, Sijin Guo, Hui Li, Kaiming Zhang, Congcong Xu, Wah Chiu, Peixuan Guo (2021) Regulation of reversible conformational change, size switching, and immunomodulation of RNA nanocubes. RNA. | Paper | Link
Kaiming Zhang, Ivan N Zheludev, Rachel J Hagey, Raphael Haslecker, Yixuan J Hou, Rachael Kretsch, Grigore D Pintilie, Ramya Rangan, Wipapat Kladwang, Shanshan Li, Marie Teng-Pei Wu, Edward A Pham, Claire Bernardin-Souibgui, Ralph S Baric, Timothy P Sheahan, Victoria D’Souza, Jeffrey S Glenn, Wah Chiu, Rhiju Das (2021) Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome. Nature Structural & Molecular Biology. | Paper | Link
Zhaoming Su, Kaiming Zhang, Kalli Kappel, Shanshan Li, Michael Z Palo, Grigore D Pintilie, Ramya Rangan, Bingnan Luo, Yuquan Wei, Rhiju Das, Wah Chiu (2021) Cryo-EM structures of full-length Tetrahymena ribozyme at 3.1 Å resolution. Nature. | Paper | Link
Yanyan Zhao, Michael F Schmid, Judith Frydman, Wah Chiu (2021) CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin. Nature Communications. | Paper | Link
Muyuan Chen, David Chmielewski, Wah Chiu, Steven Ludtke (2021) Explore the complexity of proteins with an expanded CryoET data processing pipeline. Microscopy and Microanalysis. | Paper | Link
Grigore Pintilie, Wah Chiu (2021) CryoEM Map-Model Scores: From Average Density to Q-scores. Microscopy and Microanalysis. | Paper | Link
Corey Hecksel, Kaiming Zhang, Grigore Pintilie, Patrick Mitchell, Yee-Ting Li, Wah Chiu (2021) High Resolution Data Collection at S2C2, a National CryoEM Center. Microscopy and Microanalysis. | Paper | Link
Zewen Zhang, Yi Cui, Rafael Vila, Yanbin Li, Wenbo Zhang, Weijiang Zhou, Wah Chiu, Yi Cui (2021) Cryogenic Electron Microscopy for Energy Materials. Accounts of Chemical Research. | Paper | Link
Jesús G Galaz-Montoya, Sarah H Shahmoradian, Koning Shen, Judith Frydman, Wah Chiu (2021) Cryo-electron tomography provides topological insights into mutant huntingtin exon 1 and polyQ aggregates. Communications Biology. | Paper | Link
Ugis Sarkans, Wah Chiu, Lucy Collinson, Michele C Darrow, Jan Ellenberg, David Grunwald, Jean-Karim Hériché, Andrii Iudin, Gabriel G Martins, Terry Meehan, Kedar Narayan, Ardan Patwardhan, Matthew Robert Geoffrey Russell, Helen R Saibil, Caterina Strambio-De-Castillia, Jason R Swedlow, Christian Tischer, Virginie Uhlmann, Paul Verkade, Mary Barlow, Omer Bayraktar, Ewan Birney, Cesare Catavitello, Christopher Cawthorne, Stephan Wagner-Conrad, Elizabeth Duke, Perrine Paul-Gilloteaux, Emmanuel Gustin, Maria Harkiolaki, Pasi Kankaanpää, Thomas Lemberger, Jo McEntyre, Josh Moore, Andrew W Nicholls, Shuichi Onami, Helen Parkinson, Maddy Parsons, Marina Romanchikova, Nicholas Sofroniew, Jim Swoger, Nadine Utz, Lenard M Voortman, Frances Wong, Peijun Zhang, Gerard J Kleywegt, Alvis Brazma (2021) REMBI: Recommended Metadata for Biological Images—enabling reuse of microscopy data in biology. Nature methods. | Paper | Link
Hui Li, Kaiming Zhang, Daniel W Binzel, Lyudmila S Shlyakhtenko, Yuri L Lyubchenko, Wah Chiu, Peixuan Guo (2021) RNA nanotechnology to build a dodecahedral genome of single-stranded RNA virus. RNA biology. | Paper | Link
Seyed-Fakhreddin Torabi, Yen-Lin Chen, Kaiming Zhang, Jimin Wang, Suzanne J DeGregorio, Anand T Vaidya, Zhaoming Su, Suzette A Pabit, Wah Chiu, Lois Pollack, Joan A Steitz (2021) Structural analyses of an RNA stability element interacting with poly (A). Proceedings of the National Academy of Sciences. | Paper | Link
Jonathan J Knowlton, Daniel Gestaut, Boxue Ma, Gwen Taylor, Alpay Burak Seven, Alexander Leitner, Gregory J Wilson, Sreejesh Shanker, Nathan A Yates, BV Venkataram Prasad, Ruedi Aebersold, Wah Chiu, Judith Frydman, Terence S Dermody (2021) Structural and functional dissection of reovirus capsid folding and assembly by the prefoldin-TRiC/CCT chaperone network. Proceedings of the National Academy of Sciences. | Paper | Link
Catherine L Lawson, Andriy Kryshtafovych, Paul D Adams, Pavel V Afonine, Matthew L Baker, Benjamin A Barad, Paul Bond, Tom Burnley, Renzhi Cao, Jianlin Cheng, Grzegorz Chojnowski, Kevin Cowtan, Ken A Dill, Frank DiMaio, Daniel P Farrell, James S Fraser, Mark A Herzik, Soon Wen Hoh, Jie Hou, Li-Wei Hung, Maxim Igaev, Agnel P Joseph, Daisuke Kihara, Dilip Kumar, Sumit Mittal, Bohdan Monastyrskyy, Mateusz Olek, Colin M Palmer, Ardan Patwardhan, Alberto Perez, Jonas Pfab, Grigore D Pintilie, Jane S Richardson, Peter B Rosenthal, Daipayan Sarkar, Luisa U Schäfer, Michael F Schmid, Gunnar F Schröder, Mrinal Shekhar, Dong Si, Abishek Singharoy, Genki Terashi, Thomas C Terwilliger, Andrea Vaiana, Liguo Wang, Zhe Wang, Stephanie A Wankowicz, Christopher J Williams, Martyn Winn, Tianqi Wu, Xiaodi Yu, Kaiming Zhang, Helen M Berman, Wah Chiu (2021) Cryo-EM model validation recommendations based on outcomes of the 2019 EMDataResource challenge. Nature methods. | Paper | Link
Stefan L Oliver, Yi Xing, Dong-Hua Chen, Soung Hun Roh, Grigore D Pintilie, David A Bushnell, Marvin H Sommer, Edward Yang, Andrea Carfi, Wah Chiu, Ann M Arvin (2021) The N-terminus of varicella-zoster virus glycoprotein B has a functional role in fusion. PLoS Pathogens. | Paper | Link
Zewen Zhang, Jinlong Yang, William Huang, Hansen Wang, Weijiang Zhou, Yanbin Li, Yuzhang Li, Jinwei Xu, Wenxiao Huang, Wah Chiu, Yi Cui (2021) Cathode-Electrolyte Interphase in Lithium Batteries Revealed by Cryogenic Electron Microscopy. Matter. | Paper | Link
Abigail E Powell, Kaiming Zhang, Mrinmoy Sanyal, Shaogeng Tang, Payton A Weidenbacher, Shanshan Li, Tho D Pham, John E Pak, Wah Chiu, Peter S Kim (2021) A single immunization with spike-functionalized ferritin vaccines elicits neutralizing antibody responses against SARS-CoV-2 in mice. ACS central science. | Paper | Link
Joy Hsu, Wah Chiu, Serena Yeung (2021) DARCNN: Domain Adaptive Region-based Convolutional Neural Network for Unsupervised Instance Segmentation in Biomedical Images. Proceedings of the IEEE/CVF Conference on Computer Vision and Pattern Recognition. | Paper | Link
Wah Chiu, Michael F Schmid, Grigore D Pintilie, Catherine L Lawson (2021) Evolution of standardization and dissemination of cryo-EM structures and data jointly by the community, PDB, and EMDB. Journal of Biological Chemistry. | Paper | Link
2020
Hye Ryoung Lee, Lei Liao, Wang Xiao, Arturas Vailionis, Antonio J Ricco, Robin White, Yoshio Nishi, Wah Chiu, Steven Chu, Yi Cui (2020) Three-dimensional analysis of particle distribution on filter layers inside N95 respirators by deep learning. Nano letters. | Paper | Link
Kaiming Zhang, Grigore D Pintilie, Shanshan Li, Michael F Schmid, Wah Chiu (2020) Resolving individual atoms of protein complex by cryo-electron microscopy. Cell research. | Paper | Link
Congcong Xu, Kaiming Zhang, Hongran Yin, Zhefeng Li, Alexey Krasnoslobodtsev, Zhen Zheng, Zhouxiang Ji, Sijin Guo, Shanshan Li, Wah Chiu, Peixuan Guo (2020) 3D RNA nanocage for encapsulation and shielding of hydrophobic biomolecules to improve the in vivo biodistribution. Nano Research. | Paper | Link
Gong-Her Wu, Patrick G Mitchell, Jesus G Galaz-Montoya, Corey W Hecksel, Emily M Sontag, Vimal Gangadharan, Jeffrey Marshman, David Mankus, Margaret E Bisher, Abigail KR Lytton-Jean, Judith Frydman, Kirk Czymmek, Wah Chiu (2020) Multi-scale 3D cryo-correlative microscopy for vitrified cells. Structure. | Paper | Link
Soung-Hun Roh, Mrinal Shekhar, Grigore Pintilie, Christophe Chipot, Stephan Wilkens, Abhishek Singharoy, Wah Chiu (2020) Cryo-EM and MD infer water-mediated proton transport and autoinhibition mechanisms of Vo complex. Science advances. | Paper | Link
Lisa Selzer, Zhaoming Su, Grigore D Pintilie, Wah Chiu, Karla Kirkegaard (2020) Full-length three-dimensional structure of the influenza A virus M1 protein and its organization into a matrix layer. PLoS biology. | Paper | Link
Rafael K Campos, Jing Jin, Grace H Rafael, Mervin Zhao, Lei Liao, Graham Simmons, Steven Chu, Scott C Weaver, Wah Chiu, Yi Cui (2020) Decontamination of SARS-CoV-2 and other RNA viruses from N95 level meltblown polypropylene fabric using heat under different humidities. ACS nano. | Paper | Link
Aaron M Muscarella, Wei Dai, Patrick G Mitchell, Weijie Zhang, Hai Wang, Luyu Jia, Fabio Stossi, Michael A Mancini, Wah Chiu, Xiang H-F Zhang (2020) Unique cellular protrusions mediate breast cancer cell migration by tethering to osteogenic cells. NPJ breast cancer. | Paper | Link
Peter D Dahlberg, Davis Perez, Zhaoming Su, Wah Chiu, WE Moerner (2020) Cryogenic Correlative Single‐Particle Photoluminescence Spectroscopy and Electron Tomography for Investigation of Nanomaterials. Angewandte Chemie International Edition. | Paper | Link
Kaiming Zhang, Grigore D Pintilie, Shanshan Li, Michael F Schmid, Wah Chiu (2020) Resolving Individual-Atom of Protein Complex by Cryo-Electron Microscopy. Cell Research. | Paper | Link
Stefan L Oliver, Yi Xing, Dong-Hua Chen, Soung Hun Roh, Grigore D Pintilie, David A Bushnell, Marvin H Sommer, Edward Yang, Andrea Carfi, Wah Chiu, Ann M Arvin (2020) A glycoprotein B-neutralizing antibody structure at 2.8 Å uncovers a critical domain for herpesvirus fusion initiation. Nature communications. | Paper | Link
Benjamin Bammes, Weijiang Zhou, Wah Chiu (2020) Sub-Ångström-resolution MicroED Using a Direct Detection Camera. Microscopy and Microanalysis. | Paper | Link
Yanbin Li, William Huang, Yuzhang Li, Wah Chiu, Yi Cui (2020) Opportunities for Cryogenic Electron Microscopy in Materials Science and Nanoscience. ACS nano. | Paper | Link
Kalli Kappel, Kaiming Zhang, Zhaoming Su, Andrew M Watkins, Wipapat Kladwang, Shanshan Li, Grigore Pintilie, Ved V Topkar, Ramya Rangan, Ivan N Zheludev, Joseph D Yesselman, Wah Chiu, Rhiju Das (2020) Accelerated cryo-EM-guided determination of three-dimensional RNA-only structures. Nature methods. | Paper | Link
Peter D Dahlberg, Saumya Saurabh, Annina M Sartor, Jiarui Wang, Patrick G Mitchell, Wah Chiu, Lucy Shapiro, WE Moerner (2020) Cryogenic single-molecule fluorescence annotations for electron tomography reveal in situ organization of key proteins in Caulobacter. Proceedings of the National Academy of Sciences. | Paper | Link
Jason T Kaelber, Wen Jiang, Scott C Weaver, Albert J Auguste, Wah Chiu (2020) Arrangement of the polymerase complexes inside a nine-segmented dsRNA virus. Structure. | Paper | Link
Ching-Shin Huang, Xinchao Yu, Preston Fordstrom, Kaylee Choi, Ben C Chung, Soung-Hun Roh, Wah Chiu, Mingyue Zhou, Xiaoshan Min, Zhulun Wang (2020) Cryo-EM structures of NPC1L1 reveal mechanisms of cholesterol transport and ezetimibe inhibition. Science Advances. | Paper | Link
Alexander C Partin, Kaiming Zhang, Byung-Cheon Jeong, Emily Herrell, Shanshan Li, Wah Chiu, Yunsun Nam (2020) Cryo-EM structures of human Drosha and DGCR8 in complex with primary microRNA. Molecular cell. | Paper | Link
Yuzhang Li, Kecheng Wang, Weijiang Zhou, Yanbin Li, Rafael Vila, William Huang, Hongxia Wang, Guangxu Chen, Gong-Her Wu, Yuchi Tsao, Hansen Wang, Robert Sinclair, Wah Chiu, Yi Cui (2020) Cryo-EM Structures of Atomic Surfaces and Host-Guest Chemistry in Metal-Organic Frameworks. Matter. | Paper | Link
Kaiming Zhang, Shuo Wang, Shanshan Li, Yuwei Zhu, Grigore D Pintilie, Tung-Chung Mou, Michael F Schmid, Zhiwei Huang, Wah Chiu (2020) Inhibition mechanisms of AcrF9, AcrF8, and AcrF6 against type IF CRISPR–Cas complex revealed by cryo-EM. Proceedings of the National Academy of Sciences. | Paper | Link
Pintilie, G., Zhang, K., Su, Z., Li, S., Schmid, M. F., Chiu, W. (2020) Measurement of atom resolvability in cryo-EM maps with Q-scores. Nature methods. | Paper | Link
2019
Li, S., Su, Z., Lehmann, J., Stamatopoulou, V., Giarimoglou, N., Henderson, F. E., Fan, L., Pintilie, G. D., Zhang, K., Chen, M., Ludtke, S. J., Wang, Y., Stathopoulos, C., Chiu, W., Zhang, J. (2019) Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions. Nature structural & molecular biology. | Paper | Link
More Publications on Google Scholar
Chiu Lab Publications
2024
Stella Y Sun, Li-Av Segev-Zarko, Grigore D Pintilie, Chi Yong Kim, Sophia R Staggers, Michael F Schmid, Elizabeth S Egan, Wah Chiu, John C Boothroyd (10 April 2024) Cryogenic electron tomography reveals novel structures in the apical complex of Plasmodium falciparum. mBio | Paper | Link |
David Chmielewski, Guan-Chin Su, Jason T Kaelber, Grigore D Pintilie, Muyuan Chen, Jing Jin, Albert J Auguste, Wah Chiu (07 March 2024) Cryogenic electron microscopy and tomography reveal imperfect icosahedral symmetry in alphaviruses. PNAS Nexus | Paper | Link |
Rachael C Kretsch, Lily Xu, Ivan N Zheludev, Rhiju Das (01 March 2024) Tertiary folds of the SL5 RNA from the 5’ proximal region of SARS-CoV-2 and related coronaviruses. PNAS | Paper | Link |
Mengyuan Xu, Torben Neelands, Alexander S Powers, Yan Liu, Steven D Miller, Grigore D Pintilie, J Du Bois, Ron O Dror, Wah Chiu, Merritt Maduke (12 February 2024) CryoEM structures of the human CLC-2 voltage-gated chloride channel reveal a ball-and-chain gating mechanism. Elife | Paper | Link |
2023
Momei Zhou, Benjamin Vollmer, Emily Machala, Muyuan Chen, Kay Grunewald, Ann M. Arvin, Wah Chiu, Stefan L. Oliver (02 December 2023) Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states. Nature Communications | Paper | Link |
Muyuan Chen, Michael F. Schmid, Wah Chiu (16 November 2023) Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models. Nature Methods | Paper | Link |
David Chmielewski, Eric A. Wilson, Grigore Pintilie, Peng Zhao, Muyuan Chen, Michael F. Schmid, Graham Simmons, Lance Wells, Jing Jin, Abhishek Singharoy, Wah Chiu (07 November 2023) Structural insights into the modulation of coronavirus spike tilting and infectivity by hinge glycans. Nature Communications | Paper | Link |
Martin A. Walsh, Brent L. Nannenga, Tamir Gonen, Patrick M. Sexton, Denise Wooten, Wah Chiu, Fei Sun, Bridget Carragher, Clinton S. Potter, David Agard, Stephen K. Burley (2 November 2023) The next decade in structural biology. Structure | Paper | LInk |
Rachael C. Kretsch, Ebbe S. Andersen, Janusz M. Bujnicki, Wah Chiu, Rhiju Das, Bingnan Luo, Benoit Masquida, Ewan K.S. McRae, Griffin M. Schroeder, Zhaoming Su, Joseph E. Wedekind, Lily Xu, Kaiming Zhang, Ivan N. Zheludev, John Moult, Andriy Kryshtafovych (19 July 2023) RNA target highlights in CASP15: Evaluation of predicted models by structure providers. Proteins | Paper | Link |
Hirenkumar R Patel, Joel Imventarza, Ajay Kumar, Ali Shariati, Chinyere A Iweka, Aditi Swarup, Karanvir Kaushal, Shweta Modgil, Shou Li, Michael Nahmou, Gong-Her Wu, Wah Chiu, Yaping J. Liao (June 2023) Development of human cellular model for ectopic calcification to study the physiopathological mechanism for Optic Disc Drusen (ODD). Investigative Ophthalmology & Visual Science | Link
Natasha K Dudek, Jesus G Galaz-Montoya, Handuo Shi, Megan Mayer, Cristina Danita, Arianna I Celis, Tobias Viehboeck, Gong-Her Wu, Barry Behr, Silvia Bulgheresi, Kerwyn Casey Huang, Wah Chiu, David A Relman (13 April 2023) Previously uncharacterized rectangular bacterial structures in the dolphin mouth. Nature Communications | Paper | Link |
Gong-Her Wu, Chharlene Smith-Geater, Jesús G Galaz-Montoya, Yingli Gu, Sanket R Gupte, Ranen Aviner, Patrick G Mitchell, Joy Hsu, Ricardo Miramontes, Keona Q Wang, Nicolette R Geller, Cathy Hou, Cristina Danita, Lydia-Marie Joubert, Michael F Schmid, Serena Yeung, Judith Frydman, William Mobley, Chengbiao Wu, Leslie M Thompson, Wah Chiu (8 February 2023) CryoET reveals organelle phenotypes in huntington disease patient iPSC-derived and mouse primary neurons. Nature Communications. | Paper | Link
Molly F Parsons, Matthew F Allan, Shanshan Li, Tyson R Shepherd, Sakul Ratanalert, Kaiming Zhang, Krista M Pullen, Wah Chiu, Silvi Rouskin, Mark Bathe (24 January 2023) 3D RNA-scaffolded wireframe origami. Nature Communications. | Paper | Link
Shanshan Li, Kaiming Zhang, Wah Chiu (2023) Near-Atomic Resolution Cryo-EM Image Reconstruction of RNA. Methods in Molecular Biology. | Paper | Link
2022
Daniel Gestaut, Yanyan Zhao, Junsun Park, Boxue Ma, Alexander Leitner, Miranda Collier, Grigore Pintilie, Soung-Hun Roh, Wah Chiu, Judith Frydman (8 December 2022) Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT. Cell. | Press Release | Paper | Link
Stephen K Burley, Helen M Berman, Wah Chiu, Wei Dai, Justin W Flatt, Brian P Hudson, Jason T Kaelber, Sagar D Khare, Arkadiusz W Kulczyk, Catherine L Lawson, Grigore D Pintilie, Andrej Sali, Brinda Vallat, John D Westbrook, Jasmine Y Young, Christine Zardecki (2 December 2022) Electron microscopy holdings of the Protein Data Bank: the impact of the resolution revolution, new validation tools, and implications for the future. Biophysical Reviews. | Paper | Link |
Jason T Kaelber, David Chmielewski, Wah Chiu, Albert J Auguste (27 November 2022) Alphavirus Particles Can Assemble with an Alternate Triangulation Number. Viruses | Paper | Link |
Shanshan Li, Kaiming Zhang, Wah Chiu (14 October 2022) Near-Atomic Resolution Cryo-EM Image Reconstruction of RNA. Methods in Molecular Biology | Paper | Link |
Peter D Dahlberg, Davis Perez, Corey W Hecksel, Wah Chiu, W E Moerner (1 October 2022) Metallic support films reduce optical heating in cryogenic correlative light and electron tomography. Journal of Structural Biology | Paper | Link |
Cristina Danita, Wah Chiu, Jesus G Galaz-Montoya (16 September 2022) Efficient manual annotation of cryogenic electron tomograms using IMOD. STAR Protocols. | Paper | Link
Shanshan Li, Michael Z Palo, Grigore Pintilie, Xaiojing Zhang, Zhaoming Su, Kalli Kappel, Wah Chiu, Kaiming Zhang, Rhiju Das (6 September 2022) Topological crossing in the misfolded Tetrahymena ribozyme resolved by cryo-EM. Proceedings of the National Academy of Sciences. | Paper | Link
Li-av Segev-Zarko, Peter D Dahlberg, Stella Y Sun, Daniel M Pelt, Chi Yong Kim, Elizabeth S Egan, James A Sethian, Wah Chiu, John C Boothroyd (4 September 2022) Cryo-electron tomography with mixed-scale dense neural networks reveals key steps in deployment of Toxoplasma invasion machinery. PNAS Nexus | Paper | Link |
Abigail E Powell, Duo Xu, Gillie A Roth, Kaiming Zhang, Wah Chiu, Eric A Appel, Peter S Kim (24 August 2022) Multimerization of Ebola GPΔmucin on protein nanoparticle vaccines has minimal effect on elicitation of neutralizing antibodies. Frontiers in Immunology. | Paper | Link |
David Chmielewski, Michael F Schmid, Graham Simmons, Jing Jin, Wah Chiu (30 June 2022) Chikungunya virus assembly and budding visualized in situ using cryogenic electron tomography. Nature Microbiology. | Paper | Link
Xiao Wang, Shanshan Li, Hyungmin Jun, Torsten John, Kaiming Zhang, Hannah Fowler, Jonathan PK Doye, Wah Chiu, Mark Bathe (20 May 2022) Planar 2D wireframe DNA origami. Science advances. | Paper | Link
Wesley M Tierney, Ian A Vicino, Stella Y Sun, Wah Chiu, Esteban A Engel, Matthew P Taylor, Ian B Hogue (13 April 2022) Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses. Methods in Molecular Biology | Paper | Link
Yan Wang, Jennifer M Binning, Grigore D Pintilie, Wah Chiu, Gaya K Amarasinghe, Daisy W Leung, Zhaoming Su (18 March 2022) Cryo-EM analysis of Ebola virus nucleocapsid-like assembly. STAR protocols. | Paper | Link
Ashwin Balaji, Peter D Dahlberg, Li-av Segev-Zarko, Stella Sun, Wah Chiu, John Boothroyd, William E Moerner (11 February 2022) Characterizing the distribution of myosin H in the apical complex of conoid protruded and conoid retracted Toxoplasma gondii. Biophysical Journal. | Paper | Link
Kaiming Zhang, Naoki Horikoshi, Shanshan Li, Alexander S Powers, Mikhail A Hameedi, Grigore D Pintilie, Hee-Don Chae, Yousuf A Khan, Carl-Mikael Suomivuori, Ron O Dror, Kathleen M Sakamoto, Wah Chiu, Soichi Wakatsuki (7 February 2022) Cryo-EM, Protein Engineering, and Simulation Enable the Development of Peptide Therapeutics against Acute Myeloid Leukemia. American Chemical Society. | Paper | Link
Stella Y Sun, Li-av Segev-Zarko, Muyuan Chen, Grigore D Pintilie, Michael F Schmid, Steven J Ludtke, John C Boothroyd, Wah Chiu (4 February 2022) Cryo-ET of Toxoplasma parasites gives subnanometer insight into tubulin-based structures. Proceedings of the National Academy of Sciences. | Paper | Link
Zewen Zhang, Yuzhang Li, Rong Xu, Weijiang Zhou, Yanbin Li, Solomon T Oyakhire, Yecun Wu, Jinwei Xu, Hansen Wang, Zhiao Yu, David T Boyle, William Huang, Yusheng Ye, Hao Chen, Jiayu Wan, Zhenan Bao, Wah Chiu, Yi Cui (6 January 2022) Capturing the swelling of solid-electrolyte interphase in lithium metal batteries. Science. | Paper | Link
Weijiang Zhou, Benjamin Bammes, Patrick G Mitchell, Kerry Betz, Wah Chiu (January 2022) Electron crystallography of chiral and non-chiral small molecules. Ultramicroscopy. | Paper | Link
2021
Hyungmin Jun, Xiao Wang, Molly F Parsons, William P Bricker, Torsten John, Shanshan Li, Steve Jackson, Wah Chiu, Mark Bathe (2021) Rapid prototyping of arbitrary 2D and 3D wireframe DNA origami. Nucleic Acids Research. | Paper | Link
Grigore Pintilie, Wah Chiu (2021) Validation, analysis and annotation of cryo-EM structures. Acta Crystallographica Section D: Structural Biology. | Paper | Link
Peter J Blanco Carcache, Sijin Guo, Hui Li, Kaiming Zhang, Congcong Xu, Wah Chiu, Peixuan Guo (2021) Regulation of reversible conformational change, size switching, and immunomodulation of RNA nanocubes. RNA. | Paper | Link
Kaiming Zhang, Ivan N Zheludev, Rachel J Hagey, Raphael Haslecker, Yixuan J Hou, Rachael Kretsch, Grigore D Pintilie, Ramya Rangan, Wipapat Kladwang, Shanshan Li, Marie Teng-Pei Wu, Edward A Pham, Claire Bernardin-Souibgui, Ralph S Baric, Timothy P Sheahan, Victoria D’Souza, Jeffrey S Glenn, Wah Chiu, Rhiju Das (2021) Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome. Nature Structural & Molecular Biology. | Paper | Link
Zhaoming Su, Kaiming Zhang, Kalli Kappel, Shanshan Li, Michael Z Palo, Grigore D Pintilie, Ramya Rangan, Bingnan Luo, Yuquan Wei, Rhiju Das, Wah Chiu (2021) Cryo-EM structures of full-length Tetrahymena ribozyme at 3.1 Å resolution. Nature. | Paper | Link
Yanyan Zhao, Michael F Schmid, Judith Frydman, Wah Chiu (2021) CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin. Nature Communications. | Paper | Link
Muyuan Chen, David Chmielewski, Wah Chiu, Steven Ludtke (2021) Explore the complexity of proteins with an expanded CryoET data processing pipeline. Microscopy and Microanalysis. | Paper | Link
Grigore Pintilie, Wah Chiu (2021) CryoEM Map-Model Scores: From Average Density to Q-scores. Microscopy and Microanalysis. | Paper | Link
Corey Hecksel, Kaiming Zhang, Grigore Pintilie, Patrick Mitchell, Yee-Ting Li, Wah Chiu (2021) High Resolution Data Collection at S2C2, a National CryoEM Center. Microscopy and Microanalysis. | Paper | Link
Zewen Zhang, Yi Cui, Rafael Vila, Yanbin Li, Wenbo Zhang, Weijiang Zhou, Wah Chiu, Yi Cui (2021) Cryogenic Electron Microscopy for Energy Materials. Accounts of Chemical Research. | Paper | Link
Jesús G Galaz-Montoya, Sarah H Shahmoradian, Koning Shen, Judith Frydman, Wah Chiu (2021) Cryo-electron tomography provides topological insights into mutant huntingtin exon 1 and polyQ aggregates. Communications Biology. | Paper | Link
Ugis Sarkans, Wah Chiu, Lucy Collinson, Michele C Darrow, Jan Ellenberg, David Grunwald, Jean-Karim Hériché, Andrii Iudin, Gabriel G Martins, Terry Meehan, Kedar Narayan, Ardan Patwardhan, Matthew Robert Geoffrey Russell, Helen R Saibil, Caterina Strambio-De-Castillia, Jason R Swedlow, Christian Tischer, Virginie Uhlmann, Paul Verkade, Mary Barlow, Omer Bayraktar, Ewan Birney, Cesare Catavitello, Christopher Cawthorne, Stephan Wagner-Conrad, Elizabeth Duke, Perrine Paul-Gilloteaux, Emmanuel Gustin, Maria Harkiolaki, Pasi Kankaanpää, Thomas Lemberger, Jo McEntyre, Josh Moore, Andrew W Nicholls, Shuichi Onami, Helen Parkinson, Maddy Parsons, Marina Romanchikova, Nicholas Sofroniew, Jim Swoger, Nadine Utz, Lenard M Voortman, Frances Wong, Peijun Zhang, Gerard J Kleywegt, Alvis Brazma (2021) REMBI: Recommended Metadata for Biological Images—enabling reuse of microscopy data in biology. Nature methods. | Paper | Link
Hui Li, Kaiming Zhang, Daniel W Binzel, Lyudmila S Shlyakhtenko, Yuri L Lyubchenko, Wah Chiu, Peixuan Guo (2021) RNA nanotechnology to build a dodecahedral genome of single-stranded RNA virus. RNA biology. | Paper | Link
Seyed-Fakhreddin Torabi, Yen-Lin Chen, Kaiming Zhang, Jimin Wang, Suzanne J DeGregorio, Anand T Vaidya, Zhaoming Su, Suzette A Pabit, Wah Chiu, Lois Pollack, Joan A Steitz (2021) Structural analyses of an RNA stability element interacting with poly (A). Proceedings of the National Academy of Sciences. | Paper | Link
Jonathan J Knowlton, Daniel Gestaut, Boxue Ma, Gwen Taylor, Alpay Burak Seven, Alexander Leitner, Gregory J Wilson, Sreejesh Shanker, Nathan A Yates, BV Venkataram Prasad, Ruedi Aebersold, Wah Chiu, Judith Frydman, Terence S Dermody (2021) Structural and functional dissection of reovirus capsid folding and assembly by the prefoldin-TRiC/CCT chaperone network. Proceedings of the National Academy of Sciences. | Paper | Link
Catherine L Lawson, Andriy Kryshtafovych, Paul D Adams, Pavel V Afonine, Matthew L Baker, Benjamin A Barad, Paul Bond, Tom Burnley, Renzhi Cao, Jianlin Cheng, Grzegorz Chojnowski, Kevin Cowtan, Ken A Dill, Frank DiMaio, Daniel P Farrell, James S Fraser, Mark A Herzik, Soon Wen Hoh, Jie Hou, Li-Wei Hung, Maxim Igaev, Agnel P Joseph, Daisuke Kihara, Dilip Kumar, Sumit Mittal, Bohdan Monastyrskyy, Mateusz Olek, Colin M Palmer, Ardan Patwardhan, Alberto Perez, Jonas Pfab, Grigore D Pintilie, Jane S Richardson, Peter B Rosenthal, Daipayan Sarkar, Luisa U Schäfer, Michael F Schmid, Gunnar F Schröder, Mrinal Shekhar, Dong Si, Abishek Singharoy, Genki Terashi, Thomas C Terwilliger, Andrea Vaiana, Liguo Wang, Zhe Wang, Stephanie A Wankowicz, Christopher J Williams, Martyn Winn, Tianqi Wu, Xiaodi Yu, Kaiming Zhang, Helen M Berman, Wah Chiu (2021) Cryo-EM model validation recommendations based on outcomes of the 2019 EMDataResource challenge. Nature methods. | Paper | Link
Stefan L Oliver, Yi Xing, Dong-Hua Chen, Soung Hun Roh, Grigore D Pintilie, David A Bushnell, Marvin H Sommer, Edward Yang, Andrea Carfi, Wah Chiu, Ann M Arvin (2021) The N-terminus of varicella-zoster virus glycoprotein B has a functional role in fusion. PLoS Pathogens. | Paper | Link
Zewen Zhang, Jinlong Yang, William Huang, Hansen Wang, Weijiang Zhou, Yanbin Li, Yuzhang Li, Jinwei Xu, Wenxiao Huang, Wah Chiu, Yi Cui (2021) Cathode-Electrolyte Interphase in Lithium Batteries Revealed by Cryogenic Electron Microscopy. Matter. | Paper | Link
Abigail E Powell, Kaiming Zhang, Mrinmoy Sanyal, Shaogeng Tang, Payton A Weidenbacher, Shanshan Li, Tho D Pham, John E Pak, Wah Chiu, Peter S Kim (2021) A single immunization with spike-functionalized ferritin vaccines elicits neutralizing antibody responses against SARS-CoV-2 in mice. ACS central science. | Paper | Link
Joy Hsu, Wah Chiu, Serena Yeung (2021) DARCNN: Domain Adaptive Region-based Convolutional Neural Network for Unsupervised Instance Segmentation in Biomedical Images. Proceedings of the IEEE/CVF Conference on Computer Vision and Pattern Recognition. | Paper | Link
Wah Chiu, Michael F Schmid, Grigore D Pintilie, Catherine L Lawson (2021) Evolution of standardization and dissemination of cryo-EM structures and data jointly by the community, PDB, and EMDB. Journal of Biological Chemistry. | Paper | Link
2020
Hye Ryoung Lee, Lei Liao, Wang Xiao, Arturas Vailionis, Antonio J Ricco, Robin White, Yoshio Nishi, Wah Chiu, Steven Chu, Yi Cui (2020) Three-dimensional analysis of particle distribution on filter layers inside N95 respirators by deep learning. Nano letters. | Paper | Link
Kaiming Zhang, Grigore D Pintilie, Shanshan Li, Michael F Schmid, Wah Chiu (2020) Resolving individual atoms of protein complex by cryo-electron microscopy. Cell research. | Paper | Link
Congcong Xu, Kaiming Zhang, Hongran Yin, Zhefeng Li, Alexey Krasnoslobodtsev, Zhen Zheng, Zhouxiang Ji, Sijin Guo, Shanshan Li, Wah Chiu, Peixuan Guo (2020) 3D RNA nanocage for encapsulation and shielding of hydrophobic biomolecules to improve the in vivo biodistribution. Nano Research. | Paper | Link
Gong-Her Wu, Patrick G Mitchell, Jesus G Galaz-Montoya, Corey W Hecksel, Emily M Sontag, Vimal Gangadharan, Jeffrey Marshman, David Mankus, Margaret E Bisher, Abigail KR Lytton-Jean, Judith Frydman, Kirk Czymmek, Wah Chiu (2020) Multi-scale 3D cryo-correlative microscopy for vitrified cells. Structure. | Paper | Link
Soung-Hun Roh, Mrinal Shekhar, Grigore Pintilie, Christophe Chipot, Stephan Wilkens, Abhishek Singharoy, Wah Chiu (2020) Cryo-EM and MD infer water-mediated proton transport and autoinhibition mechanisms of Vo complex. Science advances. | Paper | Link
Lisa Selzer, Zhaoming Su, Grigore D Pintilie, Wah Chiu, Karla Kirkegaard (2020) Full-length three-dimensional structure of the influenza A virus M1 protein and its organization into a matrix layer. PLoS biology. | Paper | Link
Rafael K Campos, Jing Jin, Grace H Rafael, Mervin Zhao, Lei Liao, Graham Simmons, Steven Chu, Scott C Weaver, Wah Chiu, Yi Cui (2020) Decontamination of SARS-CoV-2 and other RNA viruses from N95 level meltblown polypropylene fabric using heat under different humidities. ACS nano. | Paper | Link
Aaron M Muscarella, Wei Dai, Patrick G Mitchell, Weijie Zhang, Hai Wang, Luyu Jia, Fabio Stossi, Michael A Mancini, Wah Chiu, Xiang H-F Zhang (2020) Unique cellular protrusions mediate breast cancer cell migration by tethering to osteogenic cells. NPJ breast cancer. | Paper | Link
Peter D Dahlberg, Davis Perez, Zhaoming Su, Wah Chiu, WE Moerner (2020) Cryogenic Correlative Single‐Particle Photoluminescence Spectroscopy and Electron Tomography for Investigation of Nanomaterials. Angewandte Chemie International Edition. | Paper | Link
Kaiming Zhang, Grigore D Pintilie, Shanshan Li, Michael F Schmid, Wah Chiu (2020) Resolving Individual-Atom of Protein Complex by Cryo-Electron Microscopy. Cell Research. | Paper | Link
Stefan L Oliver, Yi Xing, Dong-Hua Chen, Soung Hun Roh, Grigore D Pintilie, David A Bushnell, Marvin H Sommer, Edward Yang, Andrea Carfi, Wah Chiu, Ann M Arvin (2020) A glycoprotein B-neutralizing antibody structure at 2.8 Å uncovers a critical domain for herpesvirus fusion initiation. Nature communications. | Paper | Link
Benjamin Bammes, Weijiang Zhou, Wah Chiu (2020) Sub-Ångström-resolution MicroED Using a Direct Detection Camera. Microscopy and Microanalysis. | Paper | Link
Yanbin Li, William Huang, Yuzhang Li, Wah Chiu, Yi Cui (2020) Opportunities for Cryogenic Electron Microscopy in Materials Science and Nanoscience. ACS nano. | Paper | Link
Kalli Kappel, Kaiming Zhang, Zhaoming Su, Andrew M Watkins, Wipapat Kladwang, Shanshan Li, Grigore Pintilie, Ved V Topkar, Ramya Rangan, Ivan N Zheludev, Joseph D Yesselman, Wah Chiu, Rhiju Das (2020) Accelerated cryo-EM-guided determination of three-dimensional RNA-only structures. Nature methods. | Paper | Link
Peter D Dahlberg, Saumya Saurabh, Annina M Sartor, Jiarui Wang, Patrick G Mitchell, Wah Chiu, Lucy Shapiro, WE Moerner (2020) Cryogenic single-molecule fluorescence annotations for electron tomography reveal in situ organization of key proteins in Caulobacter. Proceedings of the National Academy of Sciences. | Paper | Link
Jason T Kaelber, Wen Jiang, Scott C Weaver, Albert J Auguste, Wah Chiu (2020) Arrangement of the polymerase complexes inside a nine-segmented dsRNA virus. Structure. | Paper | Link
Ching-Shin Huang, Xinchao Yu, Preston Fordstrom, Kaylee Choi, Ben C Chung, Soung-Hun Roh, Wah Chiu, Mingyue Zhou, Xiaoshan Min, Zhulun Wang (2020) Cryo-EM structures of NPC1L1 reveal mechanisms of cholesterol transport and ezetimibe inhibition. Science Advances. | Paper | Link
Alexander C Partin, Kaiming Zhang, Byung-Cheon Jeong, Emily Herrell, Shanshan Li, Wah Chiu, Yunsun Nam (2020) Cryo-EM structures of human Drosha and DGCR8 in complex with primary microRNA. Molecular cell. | Paper | Link
Yuzhang Li, Kecheng Wang, Weijiang Zhou, Yanbin Li, Rafael Vila, William Huang, Hongxia Wang, Guangxu Chen, Gong-Her Wu, Yuchi Tsao, Hansen Wang, Robert Sinclair, Wah Chiu, Yi Cui (2020) Cryo-EM Structures of Atomic Surfaces and Host-Guest Chemistry in Metal-Organic Frameworks. Matter. | Paper | Link
Kaiming Zhang, Shuo Wang, Shanshan Li, Yuwei Zhu, Grigore D Pintilie, Tung-Chung Mou, Michael F Schmid, Zhiwei Huang, Wah Chiu (2020) Inhibition mechanisms of AcrF9, AcrF8, and AcrF6 against type IF CRISPR–Cas complex revealed by cryo-EM. Proceedings of the National Academy of Sciences. | Paper | Link
Pintilie, G., Zhang, K., Su, Z., Li, S., Schmid, M. F., Chiu, W. (2020) Measurement of atom resolvability in cryo-EM maps with Q-scores. Nature methods. | Paper | Link
2019
Li, S., Su, Z., Lehmann, J., Stamatopoulou, V., Giarimoglou, N., Henderson, F. E., Fan, L., Pintilie, G. D., Zhang, K., Chen, M., Ludtke, S. J., Wang, Y., Stathopoulos, C., Chiu, W., Zhang, J. (2019) Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions. Nature structural & molecular biology. | Paper | Link